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(JA /
& 0.001 #177; 1.048
æ 0.002 #177; 0.138
=
IC50)
ã #1Ç
=
0.222
=
0.415
IC50)
cytochrome c Ú ~ÑÇ +", ~~~ 10
~~ ãÑ~0æÑ~"9~Ç c111-" ì.4
ã #~Ç N"#$%Ç &~ Ê ~~J L~~Ç N"#~J.4
Ú_~1.
L.s.4.L' 441a
J.z141Ç z. ~11Ç äÅ
. yo.:1Ç +",
(JA /
æ 0.015 #177;
@~ 0.084 #177; 2.68
&~'~(Ç P 40:1.1***
calÇ ÖÇj."Ç ÌD, 0
"41..lÇ Ê 4ÂJAIÇ ~3å á %1Ç ~~~
9U>
. ÉiZ3Ç Ñæ34Ç
.?ÇÒÅ C:11Ç-S33 t~.%1Ç
`6ÂjAlÇ
RESUME : L'objectif principal de cette
étude est l'évaluation de l'effet inhibiteur des extraits des
racines de la plante cachrys libanotis, utilisée en
médecine traditionnelle en Algérie, sur la xanthine oxydase (XO)
et de ses propriétés antioxydants. La XO a été
purifiée du lait bovin avec un rendement de17.97 mg/l,
un rapport protéine/flavine de 7.18, et avec une bande majeure d'environ
150 KDa, en SDS-PAGE, indiquant une pureté acceptable de l'enzyme.
L'activité spécifique de l'enzyme purifiée a
été de 2261 nmol/min/mg protéine. L'EAc donne la plus
puissante inhibition en vers XO avec un IC50 de (IC50 = 0.11#177;
0.006mg/ml)suivi par l'ECh et l'EBr (IC50 =0.138#177; 0.002 et
1.048#177; 0.001mg /ml) par contre l'inhibition de la l'ECh donne la plus
grande inhibition en vers la reduction de cyt c avec un (IC50 = 0.222 #177;
0.0015mg/ml), suivi par l'EAc et l'EBr respectivement avec (0.415 #177; 0.015et
2.68 #177; 0.084mg /ml), les résultats obtenus suggèrent que ces
produits naturels peut être utiliser pour traiter les maladies qui
nécessites l'inhibition de la XO et le scavenger des radicaux libres
IC50)
SUMMARY: This study was conducted to search
for xanthine oxidase (XO) inhibitors from Cachrys libanotis
L.root extracts traditionally used in folk medicine in Algeria.
XO was purified from fresh bovine milk with yields of 17.97 mg / L, protein /
flavine ratio of 7.18 and a major band, on SDSPAGE, of approximately 150 KDa,
indicating an acceptable purity of the enzyme. The total specific activity of
the purified enzyme was of 2261 nmol / min / mg protein. The EAc showed the
highest in inhibitory properties on the XO activity (IC50 = 0.11 #177; 0.006 mg
/ ml), followed by ECh with IC50 for XO inhibitory activity of
0.138 #177; 0.002 mg / ml then EBr with an IC50 (
1.048#177;0.001 mg / ml). Whereas, the inhibition of cyt c reduction the
highest activity was that of ECh with IC50of 0.222 #177; 0.001 mg / ml),
followed by EAc then EBr extracts with an IC50 of 0.415 #177; 0.015 mg a /ml
and 2.68 #177; 0.084 mg / ml, respectively. These results suggest that these
natural products could be used to treat lot of diseases, where inhibition of XO
and free radical scavenging activity are necessary.
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