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Control E100
E200 E300
145
140
135
130
125
*
** **
**
120
**
115
*
**
** **
110
**
105
100
95
0 6 12 18 24 30
Days after extract administration
Figure 1: Body weight mass (expressed as % of
initial values iV = 100 %) over 30 days of treatment (once a day) with
methanol/methylene chloride leaves extract of T glaucescens 100 mg/kg
(NE100), 200 mg/kg (NE200), 300 mg/kg (NE300) bw. Data are mean #177; SEM, (n=7
per group). Significant difference: *P< 0.05, **P< 0.01 compared with
control values.
11
C
10
E1 00
E200
E300
**
9
8
7
6
**
**
0 153045 607590105120
Minutes after glucose administration
Figure 2: Plasma glucose levels during oral
glucose tolerance test after 28 days of treatment (once a day) with
methanol/methylene chloride leaves extract of T glaucescens 100 mg/kg
(NE100), 200 mg/kg (NE200), 300 mg/kg (NE300) bw. Data are mean #177; SEM, (n=7
per group). Significant difference: *P< 0.05, **P< 0.01 compared with
control values.
Table 1: Anthropometric parameters of mice
after 30 days of treatment (once a day) with 100 mg/kg (NE 100), 200 mg/kg
(NE200), 300 mg/kg (NE300) bw methanol/methylene
|
chloride extract of
|
T glaucescens leaves.
|
|
|
|
Control
|
NE 100
|
NE200
|
NE300
|
|
Inguinal WAT (mg)
|
16.14 #177; 1.35
|
13.64 #177; 1.49
|
12.43 #177; 0.86
|
8.23 #177; 0.60**
|
|
Retroperit WAT (mg)
|
16.57 #177; 1.51
|
14.71 #177; 0.65
|
13.29 #177; 0.60
|
11.14 #177; 0.56*
|
|
Parametrial WAT (mg)
|
38.00 #177; 2.79
|
36.71 #177; 1.68
|
21.00 #177; 0.28*
|
13.29 #177; 0.46**
|
|
Total fat (mg)
|
70.71 #177; 4.95
|
65.07 #177; 1.93
|
46.71 #177; 1.42*
|
32.66 #177; 0.75**
|
|
Liver (g)
|
2.20 #177; 0.05
|
2.17 #177; 0.03
|
2.18 #177; 0.03
|
2.19 #177; 0.03
|
|
Heart (mg)
|
124.71 #177; 2.58
|
121.57 #177; 2.52
|
120.33 #177; 2.18
|
118.67 #177; 3.35
|
|
Kidney (mg)
|
225.71 #177; 0.83
|
221.86 #177; 1.62
|
222.33 #177; 1.98
|
224.33 #177; 1.89
|
|
Carcass (g)
|
22.57 #177; 0.61
|
22.28 #177; 0.52
|
21.71 #177; 0.52
|
21.86 #177; 0.40
|
|
Length (cm)
|
9.64 #177; 0.10
|
9.57 #177; 0.09
|
9.56 #177; 0.09
|
9.54 #177; 0.08
|
|
Weight gain (g)
|
34.93 #177; 0.28
|
33.50 #177; 0.12
|
31.10 #177; 0.1 1**
|
28.55 #177; 0.16**
|
|
Lee index (x 1000)
|
340.00 #177; 8.13
|
338.00 #177; 8.67
|
332.29 #177; 8.01
|
322.43 #177; 5.65
|
|
Metabolic efficiency
|
0.074 #177; 0.007
|
0.061 #177; 0.010
|
0.042 #177; 0.01 5*
|
0.023 #177; 0.01 9*
|
Data are mean #177; SEM (n=7 per group).
Significant difference: *P< 0.05; **P< 0.01 compared with
control values.
Table 2: Mice Openfield test after treatment
(once a day during 27 days) with 100 mg/kg (NE100), 200 mg/kg (NE200), 300
mg/kg (NE300) bw methanol/methylene chloride extract of T glaucescens
leaves.
|
|
|
|
|
|
|
|
|
|
Control
|
NE100
|
NE200
|
NE300
|
|
Locomotors activity (AU)
|
137.29 #177; 19.27
|
144.28 #177; 23.85
|
159.57 #177; 19.68
|
168.29 #177; 18.98
|
|
Rearing (Nb per 2 min)
|
0.43
|
#177; 0.20
|
0.43
|
#177;0 02
|
0.43
|
#177; 0.30
|
0.71
|
#177; 0.36
|
|
Grooming (Nb per 2 min)
|
32.86
|
#177; 6.31
|
30.86
|
#177; 8.53
|
22.14
|
#177; 4.09
|
31.29
|
#177; 7.52
|
|
Defecation (Nb per 2 min)
|
0.43
|
#177; 0.30
|
0.86
|
#177; 0.46
|
0.43
|
#177; 0.30
|
0.57
|
#177; 0.30
|
Data are mean #177; SEM (n=7 per group).
Table 3: Plasma parameters levels of mice after
the treatment (once a day during 30 days) with methanol/methylene chloride
leaves extract of T glaucescens 100 mg/kg (NE 100), 200
|
mg/kg (NE200), 300
|
mg/kg (NE300) bw.
|
|
|
|
|
Control
|
NE100
|
NE200
|
NE300
|
|
Leptin (mg/L)
|
5.86 #177; 0.50
|
5.37 #177; 0.36
|
4.94 #177; 0.26
|
4.23 #177; 0.46*
|
|
Glycaemia (mmol/L)
|
6.64 #177; 0.35
|
6.42 #177; 0.42
|
6.36 #177; 0.35
|
6.03 #177; 0.26
|
|
Triglycerides (mmol/L)
|
0.91 #177; 0.03
|
0.86 #177; 0.03
|
0.78 #177; 0.03*
|
0.79 #177; 0.02*
|
|
Cholesterol (mmol/L)
|
1.33 #177; 0.08
|
1.00 #177; 0.04*
|
1.00 #177; 0.10*
|
0.83 #177; 0.11*
|
|
NEFA (mmol/L)
|
0.38 #177; 0.03
|
0.34 #177; 0.02
|
0.28 #177; 0.01*
|
0.23 #177; 0.01*
|
|
HDL-c (mmol/L)
|
0.065 #177; 0.004
|
0.068 #177; 0.002
|
-
|
0.077 #177; 0.007
|
|
LDL-c (mmol/L)
|
0.860 #177; 0.109
|
0.714 #177; 0.197
|
-
|
0.682 #177; 0.092
|
NEFA: non esterified fatty acids; HDL-c: HDL-cholesterol; LDL-c:
LDL-cholesterol.
Data are mean #177; SEM (n=7 per group). Significant difference:
*P< 0.05 compared with control values.
| 
