B) METHODS
a)
Cellular cultures
The cells of lines HL-60 and K562 are sown with the concentration
of 3x105 cells per ml and the Cos-6 cells with the concentration of 4x105 cells
per ml.
The cells HL-60 and K562 are cultivated in RPMI 1640, added
with fetal calf serum (SVF, décomplémenté by heat (20 min.
with 56°C)) respectively with 15 and 10%, of glutamine 2 mm and
Penicillin/Streptomycine with 100 ug/ml. The cells are maintained in
exponential phase of growth by a dilution, twice by weeks.
The adherent cells Cos-6 are cultivated in DMEM, added
with 6% with SVF, glutamine 2 mm and Penicillin/Streptomycine with 100 ug/ml.
The cells are maintained in exponential phase of growth by a dilution, twice
per weeks, after a washing with the medium without serum and an separation by
twice an addition of 1 ml of trypsin during 5 min. with 37°C.
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