2. TLS makes party of the RANC
The effect TLS on the transcription passing by the way of
indication of AR suggests that TLS can belong to the RANC,
including/understanding the nuclear receivers. In order to determine the
membership of TLS to the RANC, the experiments of delay on freezing were
realized.
Proteinic extracts of Cos-6 transfectés by calcium
chloride with the plasmides of expression of RAR, RXR and TLS are used in the
experiments of delay on freezing.
These total proteinic extracts of Cos-6 are
préalablements checked by Western Blot after immunoblotting with
specific antibodies, of which the antione for labelled protein TLS (Figure
6).
Figure 6: Analyze by Western Blot of the proteinic
expression of Cos-6 transfectées
The technique of delay on freezing is carried out with the
total proteinic extracts tested previously in presence or absence of TLS
(Figure 7).
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