VI. Annexes
Annexe 1 - Exemple de GPCR d'Aspergillus flavus
En rouge : D Y P I H C
En bleu : E L F T V S A (Y P I)
Aligné vert : domaine transmembranaire
Séquence gprA : mating-type alpha-pheromone
receptor PreB
mtmaimdskfdpysqnltfhaadgtpfqvpvmtlndfyqyciqicinygaqfgasviifiilllltrpdkrassv
fflnggalllnmgrllchmiyfttdfvkayqyfssdysraptsayansilgvvlttlllvcietslvlqvqvvca
nlrrryrtvllcvsilvalipvglrlgymvencktivqtdtplslvwlesatnivitisicffcsifiiklgfai
hqrrrlgvrdfgpmkvifvmgcqtltvpallsilqyavsvpelnsnimtlvtislplssiwagvsltrssstens
psrgalwnrltdstgtrsnqtsstdtavamtypsnksstvcyadqssvkrqydpeqghgisvehdvsvhscqrl
TMpred prediction output for : TMPRED.3852.868.seq
Sequence: MTM...QRL length: 374
Prediction parameters: TM-helix length between 17 and 33
> STRONGLY prefered model: N-terminus outside
|
7 strong
# from
|
transmembrane helices, total score : 12943
to length score orientation
|
|
1 50
|
68
|
(19)
|
2342 o-i
|
|
2 72
|
97
|
(26)
|
1243 i-o
|
|
3 119
|
135
|
(17)
|
1630 o-i
|
|
4 158
|
174
|
(17)
|
2066 i-o
|
|
5 204
|
225
|
(22)
|
2950 o-i
|
|
6 241
|
259
|
(19)
|
1358 i-o
|
|
7 272
|
291
|
(20)
|
1354 o-i
|
36
Séquence gprB : a-pheromone receptor
PreA
msapsttpyaqaviipsfsilcmlltipplilhwknrnlpvvslicwllclnlfniinafiwpsddmdnwwngag
lcdvevkvmiasyvavpgnllcifrtlasmidtrramlvpskqqrwwnigidmlfcvivpvvaiathivyqasry
ilvgisgcvnsfdeswvslilawiwplvicliagyycslvvyrlhryrnqfgdilqasnsnlnksrfmrlfllsf
ivllailpvqtyvvyknielslpwhayswrvahgphwnkiqkipsggdaffdrwfpiasgfmlfilfgcgrdasr
mygsylrllrldrcfvrtqdsssdasrsntsgfsnsrfgllfhkawtstaetcaenpatansidrssddiekgra
sspkpqrgqnmswlkhpwsflhrplhrsstssgerilsrpnlstpsntvsanawagssqsrgssdltympgreaf
irvkrdisqeselrk
TMpred prediction output for : TMPRED.3470.7072.seq
Sequence: MSA...LRK length: 465
Prediction parameters: TM-helix length between 17 and 33
> STRONGLY prefered model: N-terminus outside
|
7 strong
# from
|
transmembrane helices, total score : 14177
to length score orientation
|
|
1 12
|
32
|
(21)
|
2204 o-i
|
|
2 39
|
57
|
(19)
|
2252 i-o
|
|
3 81
|
99
|
(19)
|
1028 o-i
|
|
4 121
|
139
|
(19)
|
1853 i-o
|
|
5 169
|
185
|
(17)
|
2568 o-i
|
|
6 220
|
240
|
(21)
|
2528 i-o
|
|
7 279
|
299
|
(21)
|
1744 o-i
|
37
Séquence gprC : conserved hypothetical
protein
Mslaqvlsvlasqddegnslarrlaytdagptldidplpsaqrkgliavtvmaflsfiatlvlllfityrlvfwr
snyaryigynqyivliynlvladlqqslafliclkwitenkieassaacflqgfwlqigdpgsglfvlaiavhtf
ilvalghklshrvfvcgvvgvwlfvailviiplaahgrfvfipsgawcwiseeyepirlwthyiwiflaefgtvc
lyaimwfqlrrrikqsailgnsqteslkrlrrvigymiiypvayivlslplaagrmataqgqtpsiaffcvagav
itssglvdvllytltrrnlilesepsrdrsynrfassvnrktdhlttitaaegkhtrtdisvlrthrhredddef
ghtvregstdnivqpsgmelaplgkvyqhttieithepaypeaessdrsskgsigdgkgpaqsarmwgr
TMpred prediction output for : TMPRED.4759.7323.seq
Sequence: MSL...WGR length: 444
Prediction parameters: TM-helix length between 17 and 33
> STRONGLY prefered model: N-terminus outside
|
7 strong # from
|
transmembrane helices, total score : 14513
to length score orientation
|
|
1 51
|
69
|
(19)
|
2740 o-i
|
|
2 81
|
109
|
(29)
|
1171 i-o
|
|
3 139
|
156
|
(18)
|
1838 o-i
|
|
4 163
|
187
|
(25)
|
2930 i-o
|
|
5 214
|
232
|
(19)
|
2148 o-i
|
|
6 258
|
279
|
(22)
|
1771 i-o
|
|
7 291
|
315
|
(25)
|
1915 o-i
|
| 
