III.1.4.2. Effect of extracts on the variation of body
weight
We also evaluate the effects of the dose 5000mg/Kg BW of our
extract on the variation of body weight and the results are presented as
follows.
Figure 13 : Effect of extracts on
variation of body weight during toxicity
From the above figure (figure 13), we observe that, the weight
gain of the test groups was reduced as compared to that of the negative control
and the difference was significant (p<0.05).
III.1.4.3. Effect of extracts on transaminases activities and
creatinine
Table XVI below represent the result of markers of toxicity
after acute toxicity.
Table XVI: Effect of extracts on markers of
toxicity (ASAT, ALAT and Creatinine)
|
Groups
|
ASAT (U/L)
|
ALAT (U/L)
|
Creatinine (mg/dl)
|
|
Control
|
8.632 #177; 0.052
|
91.956 #177; 0.262
|
31.913 #177; 0.770
|
|
FOHT (5000mg/Kg BW)
|
5.652 #177; 0.354*
|
85.651 #177; 1.097*
|
24.880 #177; 0.919*
|
|
FOHF (5000mg/Kg BW)
|
6.867 #177; 0.420*
|
80.704 #177; 0.196*
|
37.248 #177; 2.688*
|
*: significant difference between the control and the test
groups at the threshold of 0. 05;
From the above results we
notice a significant decrease in the concentration of ALT and AST in the test
groups and equally a decrease in the concentration of creatinine for the
extract FOHT. We also observe an increase in the concentration of creatinine
for the FOHF extract.
III.1.5. The effect of extracts on glycemia
III.1.5.1 Hypoglycemic effect of extracts on hyperglycemic
rats
The evaluation of hypoglycemic effect of FOHT and FOHF on
hyperglycemic rats revealed the following
results.
Table XVII:
Hypoglycemic effects of extracts on hyperglycemic rats
|
Glycemia (mg/dl)
|
|
Time
|
0 Hour
|
2 Hours
|
5 Hours
|
|
Control
|
121.200 #177;1.162
|
102.800 #177; 2.991
|
79.000 #177; 6.380
|
|
FOHT
|
116.600 #177; 1.275
|
107.200 #177; 4.615
|
89.400 #177; 0.266
|
|
FOHF
|
115.600 #177; 5.568
|
106.800 #177; 1.554
|
105.600 #177; 5.140 *
|
*: significant difference between the control and the test
groups at the threshold of 0. 05;
There was a decrease in glycemia for all groups after the
administration of extracts from time 0 hour to the 5th hour. This
decrease was not significantly different from the 0 to 2nd hour
between the control and the test groups. From the 2nd to the
5th hour, the decrease was still not significant between the control
and the FOHT group but there was a significant increase between the control and
the FOHF group. The percentage decrease for the extract FOHT was significantly
higher (8.908%) than that of the extract FOHF (5.747%).
III.1.5.2. Antihyperglycemia effects of extracts on normal
rats
The evaluation of the effect of FOHT and FOHF extracts in the
regulation of glucose intolerance in rats revealed the following results (table
XVIII).
Table XVIII: Antihyperglycemic effect of
extracts on normal rats
|
Glycemia (mg/dl)
|
|
0 min
|
30 min
|
60 min
|
120 min
|
|
Negative control
|
88.000#177;3.705
|
105.600 #177; 2.970
|
100.900 #177; 2.770
|
93.300#177;1.164
|
|
Positive control
|
82.400#177;3.173*a
|
175.400#177;3.809*a
|
111.300#177;2.958*a
|
91.100#177;2.642*a
|
|
FOHT
|
99.300 #177; 1.685b
|
124.700 #177; 2.905 b
|
141.800 #177; 5.495 b
|
98.300#177;1.824 a
|
|
FOHF
|
89.300 #177; 1.271 b
|
171.700 #177; 7.256 b
|
182.200 #177; 10.914 b
|
143.000#177;3.977 b
|
*: significant difference between the positive control and
the negative control
a and b : significant difference between the control
and the groups FOHT and FOHF at the threshold of 0,05;
The variation of glycemia during the glucose tolerance test
was significantly higher in the positive control as compared to the negative
control. Glucose level increased significantly 30 minutes after administration
in the positive control as compared to the treated groups but this glucose
level decreased significantly from 30 minutes up to 120 minutes. The percentage
of decrease was higher with the FOHT extract (21.566%) as compared to the FOHF
extract (8.208%).
| 
