Product Name

Molecular Formula

Molecular Weight

Manufacturer

Hemin chloride

C₃₄H₃₂ClFeN₄O

652.0

Sigma

Artemisinin

C₁₅H₂₂O₅

282.34

Guilin Pharmaceutical Factory

Dihydroartemisinin

C₁₅H₂₄O₅

284.35

Beijing Cotexcin New Technology Corporation

Artesunate

C₁₉H₂₈0₈

384.43

Guilin Pharmaceutical Factory

Chloroquine diphosphate

C₁₈H₂₆N₃Cl.2H₃PO₄

515.9

Sigma

Quinine

C₂₀H₂₄N₂O₂

324.4

Merck

Quinidine sulfate

(C₂₀H₂₄N₂O₂)₂.H₂SO₄

746.9

Merck

Sodium hydroxide A.R.

NaOH

40

Beijing Chemical Factory

Hydrochloric acid A.R, 36-38%, d=1.18

HCl

36.46

Beijing Chemical Factory

Sodium bicarbonate

NaHCO₃

84.1

Beijing Chemical Reagent Company

Dimethylsulfoxide

(CH₃)₂SO

78.13

Beijing Xing Jin Chemical Factory

Ethanol

CH₃CH₂OH

46.07

Beijing Yili Fine Chemical reagents Company

Propylene glycol

CH₃CHOHCH₂OH

76.10

Beijing Chemical Reagent Company

Methanol (HPLC grade)

CH₃OH

32.04

Fisher Scientific

Acetic acid

CH₃COOH

47.1

Beijing Chemical Reagent Company

Ethylene glycol

HOCH₂CH₂OH

62.07

Beijing Chemical Reagent Company

Sodium hydrogen phosphate A.R.

Na₂HPO₄.12H₂O

358.14

Beijing Yili Fine Chemical Reagents Company

Sodium dihydrogen phosphate A.R.

NaH₂PO₄.2H₂0

156.01

Beijing Yili Fine Chemical Reagents Company

Tris (hydroxymethyl)-methylamine

C₄H₁₁NO₃

121.09

Beijing Yili Fine Chemical Reagents Company

Appliance

Method of Analysis

Laboratory

Shimadzu UV -Visible 21OOS Spectrophotometer

Coupled to CPS-260 thermostat, Japan

Measurements of absorbance at the Soret band of hemin (interaction hemin-artemisinin compounds)

Tsinghua Analysis Center, Beijing

Perkin-Elmer Lambda 2 UV-Visible Spectrophotometer, Germany

Measurements of absorbance at the Soret band of hemin (interaction hemin-quinoline compounds )

Biomacromolecules Laboratory, Kinshasa University (Congo)

LS/MS/MS API 300

Column :SB-C18 Zorbax

4.6x250nm(5m), USA

Measurements of the mass of the hemin-artemisinin compounds complex

Tsinghua Analysis Center, Beijing

Agilent 1100 series LC/MSD Trap/ Column :SB-C18 Zorbax

4.6x250nm(5m), USA

Measurements of the mass of the hemin-artemisinin compounds complex (Analytical HPLC/DAD/MS)

Tsinghua Analysis Center, Beijing

Magnetic Stirrer 90-3

Mixture of work solutions

Chemistry Department,

Tsinghua University

Balance Adventurer, USA

Preparation of stock solutions

Chemistry Department,

Tsinghua University

Temperature Controller KWS-FIE2-B, China

Incubation at 37^oC

Tsinghua Analysis Center, Beijing

pHmeter PHS-2C(Shanghai Dapu Instruments Limited compagny) equipped with Aurora Electrode(kept soakep in 4 M KCl)

pH measurements of stock and work solutions

Chemistry Department,

Tsinghua University